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Reconfiguring the genetics of the food pathogen E. coli produces hydrocarbons indistinguishable from those burned in trucks
By David Biello
E. coli can now replicate the hydrocarbon molecules that burn predominantly in big trucks and other powerful moving machines. Image: Flickr/Carlos de Paz
Welding bits and pieces from various microbes and the camphor tree into the genetic code of Escherichia coli has allowed scientists to convince the stomach bug to produce hydrocarbons, rather than sickness or more E. coli. The gut microbe can now replicate the molecules, more commonly known as diesel, that burn predominantly in big trucks and other powerful moving machines.
"We wanted to make biofuels that could be used directly with existing engines to completely replace fossil fuels," explains biologist John Love of the University of Exeter in England, who led the research into fuels. "Our next step will be to try to develop a bacterium that could be deployed industrially." Love?s work was published April 22 in Proceedings of the National Academy of Sciences.
That means harnessing E. coli's already high tolerance for harsh conditions, such as the high acidity and warmth of the human digestive tract. That hardiness also seems to be helping the bacterium survive its own production of such longer-chain hydrocarbons, which could have proved toxic to the microbes, in the way brewer's yeast cells are killed off by the alcohol they ferment. The engineered E. coli used genetic code from the insect pathogen Photorhabdus luminescens and from the cyanobacterium Nostoc punctiforme as well as its fellow gut microbe Bacillus subtilis to make the fuel molecules from fatty acids, along with a gene from the camphor tree?Cinamomum camphora?to cut the resulting hydrocarbon to the right length.
The E. coli are currently fed on sugar and yeast extract, which suggests that the resulting fuel would be expensive compared with the kind refined from oil found in the ground. "We are hopeful that we could change their diet to something less valuable to humanity," Love suggests. "For example, organic wastes from agriculture or even sewage."
Exactly how the E. coli microbes expel the diesel fuel molecules is unknown at this point. The researchers have found them floating in the growth medium, suggesting the microbes are somehow secreting the hydrocarbons from their cells once produced. "We don't know how they get there yet," Love admits. But that may solve a problem posed to other would-be biofuels produced in microbes; algal oils have proved difficult to extract cheaply and effectively from inside the algae themselves, among other challenges.
Source: http://rss.sciam.com/click.phdo?i=d7ead1449b1788bf22e13a011d3ffafa
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The US National Institute on Aging offers tips for healthy aging. (Copyright ? 2013 HealthDay. All rights reserved.) Copyright ? 2013 HealthDay. All rights reserved. More in Fitness ? Exercise blunts effect of drinking on brain. More: FitnessNews ?
Best Prices on all YOUR Health and Fitness Requirements! CLICK HERESource: http://www.16g.org/posture-may-predict-future-disability/
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If you look at any Chinese government white paper on the future of the auto industry in China you will find that they wistfully look to ?new energy vehicles? as the end all solution to China?s car and pollution problem. New Energy includes electricity, LPG and Hydrogen amongst other flavors but the EV is the big favorite due to the storage solutions of hydrogen, not that electricity is any much easier to store. Chinese manufacturers like BYD have put out hybrid and EV models in the Chinese market but sales are so poor that they are barely making a dent, only deep pocketed companies and government agencies are buying them up, and only buying them at a slow rate.
Honda?s CEO has finally come to the conclusion that Chinese customers aren?t interested in hybrids as of yet, but he fails to come to a conclusion:
?Overall, we have high hopes for hybrid technology. In terms of how important it is to the Chinese market we are slowly releasing products and looking at how they do,? Mr. Ito told reporters in Shanghai on Saturday. ?But we think there are still more Chinese consumers who want to simply buy a car that fits their needs rather than buy a hybrid. By needs I mean a good-quality car with an affordable price that doesn?t break down. At present, we think these take higher priority.?
Perhaps Mr. Takanobu should think further along the lines as to why Chinese consumers aren?t buying hybrids from Honda or elsewhere ? and that?s because they are far more expensive than their gasoline variants. A regular gasoline Honda Fit costs circa 75,000RMB, where as the hybrid Fit is just under 180,000RMB ? the difference in running a petrol version for around 7 years or buying a larger car and having a running budget for 2 to 3 years of use.
Maserati boss Harald Wester did not share Mr. Takanobu?s enlightened views on hybrids and EV?s at the Shanghai Auto Show, he simply called all electric vehicles ?nonsense?:
Maserati boss Harald Wester has described electrically powered cars as ?nonsense?, saying regulatory bodies need to take a more realistic approach to how they measure emissions before pushing through regulations that favor them.
Citing statistics that suggest average power station efficiency means the average electric car requires 86g of CO2 to be produced to travel a kilometer in Europe, 110g/km in the USA and 191g/km in China.
Wester said: ?All this discussion about zero emissions is nonsense. Nobody talks about the efficiency of how the battery is charged. It varies strongly from region to region, depending on how the energy is produced, nuclear, coal and so on, but even the best is not ahead of the internal combustion engine.?
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ASSIUT, Egypt (AP) ? Egyptian authorities are suspending and investigating a provincial prosecutor who ordered a man flogged 80 times for public drunkenness.
Spokesman Mahmoud el-Hefnawi of the prosecutor general's office says the country's top attorney ordered Hussein Anani's decision cancelled and referred him to a judicial investigation.
Anani had cited verses from the Quran in his ruling.
Anani's decision late Sunday surprised police officers in the southern province of Minya who refused to carry out the order. Public intoxication is a criminal offense in Egypt, with penalties ranging from small fines to around three months in prison.
Egypt's penal code does not mention flogging, however.
Rights lawyer Anas Sayid Saleh says that only judges, not prosecutors, have the right to order punishments.
Source: http://news.yahoo.com/egypt-probes-prosecutor-ordered-drunk-flogged-111906607.html
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By Shilpa Jamkhandikar
MUMBAI (Reuters) - He's starred in several Bollywood blockbusters, an Oscar-winning film and played a Middle Eastern leader on the American TV series "24".
Anil Kapoor is now wooing Indian audiences with an adaptation of the hit thriller series about a counter-terrorism agent.
Kapoor, the remake's producer and lead actor, says he wanted to bring the "discipline and professionalism" of U.S. television to India.
The 53-year-old spoke to Reuters on the sets of his new show, describing his experience with the U.S. series and how "24" will hook Indian audiences like slow poison.
Q: When you did the original "24" in the U.S., how different was the experience?
A: When I went to the shoot, I was totally taken aback by the scale. My only experience of an international film at that time was "Slumdog Millionaire" which we shot in India. Now I was shooting in America ? It was much bigger than "Slumdog" - vanity vans and cars and the whole paraphernalia. It was huge. I went inside and saw the set. That day it was the U.N. set and I noticed the detailing. It was an exact replica. Exact. The detailing and everything was perfect.
I couldn't put the script down. We are not used to reading scripts (in India), we are used to narration. But I just had to finish reading this script. I thought this is like shooting a big commercial film. Everybody was well-prepared and completely immersed in the work culture. The way we shot it, the two camera set-up, the way it was. Slowly, I got completely sucked into it and started enjoying the whole process.
Q: Indian TV audiences are used to very different fare. Do you think you've taken a big risk?
A: Whatever I have done in my career - you like something, you go for it ? You stick your neck out and I have stuck my neck out. I am not playing it safe, I am playing the lead. And I'm a movie star.
Q: When you conceptualized the show, was (director) Abhinay Deo part of the plan?
A: The written material was already there, but I needed to get an international scale and feel. Then I happened to see "Delhi Belly". When I saw the performances, the pitch of the film, I said this is it. He is the director. When I met him, it turned out he was equally passionate about "24".
Q: What does it take to make an international-level TV show in India?
A: The first is the scale and the budget. Everything is about money today. I can't tell you about the budget but I can say that we are trying our best to have the same scale. That was the major thing, the biggest hurdle to cross. Convincing the channels that this is what it is.
Q: In that sense, it is an experiment. Isn't it?
A: It's not an experiment, it's a challenge. What happens is, even if the first season is good enough, that will be enough. Then the second season will come with a bang. Even in America, the first season was OK-OK. It was only after the second and third season, (it) took off. Television slowly gets into you like a poison ? They will realize that this will work like slow poison. It'll suck people into it.
Q: Do you have to add drama for the benefit of Indian audiences who aren't used to subtlety?
A: "Sholay" is a great example. The pitch is mainstream but real. You enjoy it. We are aiming for that kind of pitch and that's what I understand. The one thing Abhinay wants is for youngsters to watch television from "24". Somewhere, the footfalls in the theatres have increased and (TV) shows which were earlier successful aren't any more. There is a stagnancy. That's why they are going to theatres to watch these niche films. There are lesser sources of entertainment. The timing is very right.
Q: What if this doesn't work?
A: My wife says your body of work is so much, that it doesn't matter. You can move on. I am doing it when I am peaking. I am saying no to feature films. Big film-makers. Bigger stars than me have said yes to those roles. It doesn't work, it doesn't work. You move on.
Q: What would be the measure of success for you?
A: For me, it's already a success. That it's happening. I am already on seventh heaven. I have already celebrated.
(Editing by Vipin Das and Tony Tharakan)
Source: http://news.yahoo.com/indian-24-slow-poison-anil-kapoor-103521954.html
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